찔레뿌리 추출물의 항산화능과 인간모유두세포의 성장인자 발현에 미치는 영향 = Anti-oxidant Efficacy and Effects on Expression of Growth Factors in Human Hair Follicle Dermal Papilla Cells of Rosa multiflora Root Extracts

다국어 초록 (Multilingual Abstract)

Purpose: The anti-oxidant activity of active ingredients extracted from Rosa multiflora root was evaluated and effects of cell proliferation and growth factor expression were confirmed. Methods: Total polyphenol and flavonoid content of the active ingredients extracted from Rosa multiflora root was measured in various ways, as were 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity and nitrite scavenging activity. Further, the cell proliferation effects for human hair follicle dermal papilla cell (HFDPC) were measured using thiazolyl blue tetrazolium bromide (MTT) assay, as were the effects of promoting the expression of growth factors such as platelet derived growth factor subunit B (PDGFB), keratinocyte growth factor (KGF), insulin like growth factor 1 (IGF1), vascular endothelial growth factor (VEGF), hepatocyte growth factor (HGF), and epidermal growth factor (EGF) by Western blot. Results: The total polyphenol content of six kinds of Rosa multiflora root extracts was the highest in supercritical extracts (101.925 mg/g) and the total flavonoid content was the highest in supercritical extracts (14.365 mg/g). DPPH scavenging activity was the highest in methanol-sonication extracts (98.24%) and nitrite scavenging activity was the highest in supercritical extracts (55.86%). HFDPC was treated with 10, 100, and 500 μg/mL of Rosa multiflora root methanol-sonication extracts to determine cytotoxicity. The cell growth rate increased with increasing concentrations. The effects of the expression of growth factor protein were 135% greater in IGF1 and 126% greater in PDGFB. Conclusion: Rosa multiflora root extracts had no cytotoxicity but had excellent anti-oxidant ability. In addition, because the effects of promoting the expression of IGF1 and PDGFB were significant, it was confirmed that Rosa multiflora root extracts could be used as an antioxidant, functional cosmetic material, and as a natural material for hair restorer and a hair-growth promotion agent.

국문 초록 (Abstract)

목적: 찔레나무(Rosa multiflora) 뿌리에서 유효성분을 추출하여 항산화 활성 평가, 세포증식효과 및 성장인자 발현촉진효과를 확인하여 천연성분을 이용한 기능성 화장품 및 육모제 소재로서의 활용 가능성을 탐색하였다. 방법: 찔레나무 뿌리에서 다양한 방법으로 유효성분을 추출하여 총 폴리페놀 및 플라보노이드 함량을 측정하였고, DPPH 라디칼 소거능과 아질산염 소거능을 측정하였다. MTT assay로 인간모유두세포에 대하여 세포 독성 효과를 측정하였으며, 웨스턴블롯을 통해 모발성장인자인 PDGFB, KGF, IGF1, VEGF, HGF, EGF의 발현촉진효과를 측정하였다. 결과: 찔레뿌리 추출물 6종의 총 폴리페놀 함량은 초임계 추출물(101.925 mg/g), 총 플라보노이드 함량은 초임계 추출물(14.365 mg/g)에서 가장 높게 나타났다. DPPH 소거능은 메탄올-초음파 추출물(98.24%)에서, 아질산염 소거능은 초임계 추출물(55.86%)에서 가장 높게 나타났다. 인간모유두세포에 찔레뿌리 메탄올-초음파 추출물을 10, 100, 500 μg/mL의 농도별로 처리하여 세포 독성을 확인한 결과, 처리농도 범위 내에서 세포 독성은 관찰되지 않았으며. 농도가 증가할수록 세포 생육도가 높았다. 웨스턴블롯을 통한 성장인자 단백질의 발현효과는 대조군 대비 IGF1은 135%, PDGFB은126%로 발현촉진효과가 높게 나타났다. 결론: 찔레뿌리 추출물은 독성이 없고 항산화능이 우수하였으며, IGF1과 PDGFB에 대한발현촉진효과가 높게 나타나 항산화제, 기능성화장품, 육모제 및 발모촉진제의 천연소재로서의 활용 가능성을 확인하였다.

참고문헌 (Reference)

• 1 오희경, "흰민들레의 부위별 건조방법에 따른 영양성분 및 항산화효과 비교" 대한영양사협회 19 (19): 389-399, 2013
• 2 송미라, "환경 및 유전적 요인이 여성의 탈모에 미치는 영향" 한국디자인문화학회 16 (16): 255-261, 2010
• 3 박성진, "초고압 처리가 블루베리의 항산화 증진에 미치는 영향" 한국식품영양과학회 43 (43): 471-476, 2014
• 4 김명곤, "참마(Discorea japonica) 추출물의 생리활성 비교" 한국미용학회 19 (19): 509-515, 2013
• 5 김중학, "찔레뿌리 추출물을 주성분으로 하는 천연물 조성물의 발모촉진 효과에 관한 전임상 및 임상시험 연구" 한국약용작물학회 20 (20): 108-116, 2012
• 6 박근혜, "찔레나무뿌리(Rosa multiflora root)의 항산화 및 항염증효과" 한국생명과학회 21 (21): 1120-1126, 2011
• 7 박찬성, "쑥과 솔잎의 항산화작용 및 아질산염 소거작용" 한국식품저장유통학회 9 (9): 248-252, 2002
• 8 범희주, "수창포의　이화학적 특성과 에탄올 추출물이 UVB로부터 피부 보호에 미치는　효과" 한국피부과학연구원 8 (8): 181-194, 2010
• 9 유민정, "선학초 에탄올 추출물의 미용기능성소재 개발을 위한 연구" 한국피부과학연구원 10 (10): 757-762, 2012
• 10 이진하, "산나물류의 식품 화학적 성분과 전자 공여능" 한국약용작물학회 19 (19): 111-116, 2011
• 11 황윤경, "비파잎의 이화학적 성분과 항산화효과" 한국생명과학회 20 (20): 1625-1633, 2010
• 12 권민철, "병풀의 초음파 추출 시 용매에 따른 면역활성 증진 효과" 한국약용작물학회 16 (16): 294-300, 2008
• 13 한은경, "민들레의 부위별 에탄올 추출물의 항산화 활성" 한국식품영양과학회 40 (40): 56-62, 2011
• 14 황초롱, "미나리 에탄올 추출물의 항산화성분과 항산화활성" 한국식품영양과학회 40 (40): 316-320, 2011
• 15 Stenn KS, "The molecular and structural biology of hair" New York Academy of Sciences 11-13, 1991
• 16 Stenn KS, "The angiogenic properties of the rat vibrissa hair follicle associate with the bulb" 90 : 409-411, 1988
• 17 Mosmann T, "Rapid colorimetric assay for cellular growth and survival : application to proliferation and cytotoxicity assays" 65 : 55-63, 1983
• 18 Hertog MG, "Potential health effects of the dietary flavonol quercetin" 50 : 63-71, 1996
• 19 Hagemann T, "Positive lymphocyte transformation test in a patient with allergic contact dermatitis of the scalp after short-term use of topical minoxidil solution" 53 : 53-55, 2005
• 20 Huang MT, "Phenolic compounds in food and their effects on health Ⅱ: antioxidants and cancer prevention" American Chemical Society 54-71, 1992
• 21 Takagi S, "Lipophilic constituents of the fruits of Rosa multiflora Thunb" 100 : 466-467, 1980
• 22 Su HY, "Insulin-like growth factor 1 and hair growth" 5 : 1-, 1999
• 23 Bartsch H, "Inhibitors of endogenous nitrosation mechanisms and implications in human cancer prevention" 202 : 307-324, 1988
• 24 Gray JI, "Inhibition of N-nitrosamine formation in model food systems" 40 : 981-984, 1975
• 25 Shapiro J, "Hair regrowth : therapeutic agents" 16 : 341-356, 1998
• 26 Peters K, "Hair loss prevention through natural remedies: a prescription for healthier hair" Apple Tree Publishing 1-3, 1996
• 27 Tsuboi R, "Growth factors and hair growth" 4 : 103-108, 1997
• 28 Mitsui S, "Genes for a range of growth factors and cyclin-dependent kinase inhibitors are expressed by isolated human hair follicles" 137 : 693-698, 1997
• 29 Lin WH, "Fibroblast growth factors stimulate hair growth through β-catenin and Shh expression in C57BL/6 mice" 2015 : 730139-, 2015
• 30 Park SN, "Effect of natural products on skin cells: action and suppression of reactive oxygen species" 25 : 77-127, 1999
• 31 Randall VA, "Do androgens influence hair growth by altering the paracrine factors secreted by dermal papilla cells?" 11 : 315-320, 2001
• 32 Han JT, "Development of functional material using the root of Rosa multiflora" 11 : 59-65, 2006
• 33 Choi H, "Deoxypodophyllotoxin reduces skin pigmentation of brown Guinea pigs" 70 : 378-380, 2004
• 34 Yano K, "Control of hair growth and follicle size by VEGF-mediated angiogenesis" 107 : 409-417, 2001
• 35 류은미, "C57BL/6 마우스에서 꽃송이버섯 추출물의 에틸 아세테이트층과 물층의 분리에 따른 발모 효과" 한국피부과학연구원 9 (9): 1-10, 2011
• 36 형순희, "C57BL/6 마우스 모델에서 복합 한약추출물의 모발성장 촉진효과" 한국미용학회 13 (13): 1366-1375, 2007
• 37 Cha JY, "Antioxidative activities and contents of polyphenolic compound of Cudrania tricuspidata" 28 : 1310-1315, 1999
• 38 Shin ES, "A study on the effect of five herbal extracts on the hair growth" 2 : 91-100, 2006
• 39 Folin O, "A new (colorimetric) method for the determination of uric acid in blood" 13 : 469-475, 1913
• 40 Liu J, "5alpha-reductase inhibitory effect of triterpenoids isolated from Ganoderma lucidum" 29 : 392-395, 2006