Glycerol이 백서 좌골신경에 미치는 영향에 관한 전자현미경적 소견 = Electron Microscopic Finding of Glycerol Effects on the Sciatic Nerve of Rats

국문 초록 (Abstract)

삼차신경통 치료에 사용되는 glycerol이 말초신경섬유에 미치는 효과를 알아 보기위해 웅성백서의 우측 좌골신경에 무슨 glycerol, 좌측 좌골신경에 생리식여수를 적용하여 1주, 2주, 3주, 4주후의 신경섬유의 신경주막하 근접부위에 발생되는 조직병리학적 변화를 전자현미경으로 관찰한 결과 다음과 같은 결론을 얻었다.
실험군 1주째에 무수 glycerol로 인해 수초층의 분리현상, 수초의 종창 및 축삭의 압박현상이 관찰되었으며 실험군 2주째에서는 이러한 현상이 더욱 현저하게 일어났으며 수초의 이탈 및 축삭의 용해현상도 관찰되었다.
실험군 1주째에 소신경섬유보다 대신경섬유에서 파괴현상이 더욱 현저하게 나타났다.
실험군 1주째에 무수초 신경섬유보다 유수초 신경섬유에서 파괴현상이 더욱 현저하였다.
실험군 2주째에 schwann세포의 세포질내에 이탈된 수초가 나타났는데 이는 schwann세포의 탐식작용으로 여겨진다.
실험군 3주째에 신경섬유의 초기 재생현상이 관찰되었으며 실험군 4주째에서는 신경섬유의 재생현상이 현저하게 관찰되었는데 이는 schwann세포에 의한 신경섬유의 재생작용으로 여겨진다.

다국어 초록 (Multilingual Abstract)

The purpose of this experimental study is to investigate the effects of glycerol when anhydrous glycerol is applied topically to the sciatic nerve of rats.
For this study, 32 Sprague-Dawley male albino rats were used as the experimental animals. The right sciatic nerve of rats applied with anhydrous glycerol was used as the experimental group and the left sciatic nerve of rats applied with physiological saline was used as the control group.
Eight rats were sacrificed every first, second, third and fourth week and the sciatic nerves were removed. All specimens were fixed in 2.5% glutaraldehyde solution and post-fixed with 1% osmium tetroxide solution. The specimens were embedded in epon 812, cross-sectioned into 60nm thickness and stained with lead acetate.
After this procedures, the specimens were observed with electron microscope. The obtained results were as follows:
1. Myelin lamella separation, swelling of myelin sheath axonal compression were observed at first week and maximal destructive changes including myelin disintegration and axonolysis were noted at second week in the experimental group.
2. The destructive change was more severe in the large nerve fiber than small nerve fiber at first week in the experimental group.
3. Thd destructive change was more severe in the myelinated nerve fiber than the unmyelinated nerve fiber at first week in the experimental group.
4. The schwann cell containing disintegrated myelin was observed at second week in the experimental group, it seems to be phagocytosis of schwann cell.
5. The early restitution of nerve fiber was started at third week and the marked restitution of nerve fiber was observed at fourth week in the expermental group. This restitution of nerve fiber can be considerd as the effect of schwann cell.