정자기장과 맥동전자기장이 MC3T3-E1 세포의 ALP 및 ANA 활성동에 미치는 영향 = The Effects of Static Magnetic Field and Pulsed Electomagnetic Field on Alkaline Phospatase and DNA Synthetic Activity of MC3T3-El Cells

국문 초록 (Abstract)

정자기장과 맥동전자기장이 배양 조골세포에 미치는 영향을 알아보고자 MC3T3-E1세포를 각 자기장하에서 배양하여 ALP활성도와 DNA의 합성능을 평가한 결과 다음과 같은 결과를 얻었다.
. 정자기장을 가한 군에서 자석을 1,2,3개 가한 군 (51∼114.8mT)에서 대조군에 비하여 ALP의 유의성 있는 증가가 나타났으며 (P＜0.05), 자석을 4개 5개 가한 군 (150mT)에서는 대조군에 비하여 ALP활성도에 차이가 나타나지 않았다.
. 맥동성 전자기장에서는 대조군에 비하여 ALP활성도에 유의한 차이가 나타나지 않았다.
. DNA 합성능은 정자기장과 맥동성 전자기장을 가한군 모두 대조군에 비하여 유의한 차이가 나타나지 않았다.
이상의 결과 정자기장에 의한 교정력은 골세포의 대사과정에 변화를 줄 수 있으므로, 치아이동에 어떠한 영향을 줄 수 있을 것으로 사료된다.

다국어 초록 (Multilingual Abstract)

The purpose of this study was to evaluate the effects of magnetic field on cellular activity of MC3T3-E1 cells. The cellular activity was monitored by alkaline phosphatase and DNA synthetic activity in control, static magnetic field and pulsed electromagnetic field groups. A static magnetic field was applied to the cell by placing one, two, three,four, and five samarium-cobalt magnets above and below each cell plate for 24 hours per day. A pulsed electromagnetic field with a frequency of 100 herz was applied for 10 hours per day.
After 10 days of magnetic field exposure, there were increase of alkaline phosphatase activity in static magnetic field groups consisted of one, two and three magnetic groups.
Alkaline phosphatase activities were not significantly increased in four and five magnetic groups.
Application of pulsed electromagnetic field did not result in significant increased in alkaline phosphatase activity compared to control.
DNA synthetic activity in both static and pulsed eletromagnetic field group were ont significantly different from that in control group.
The result of this study suggest that magnetic field could have effect on the metabolism of bone cells related to the cellular metabolic process.