Mycobacterium koreense sp. nov., a slowly growing non-chromogenic species closely related to Mycobacterium triviale

다국어 초록 (Multilingual Abstract)

<P>A novel slow-growing, non-chromogenic mycobacterium (strain 01-305<SUP>T</SUP>) was isolated from a patient with pulmonary dysfunction. Growth characteristics, acid-fastness and the results of 16S rRNA gene sequencing supported the placement of this strain within the genus <I>Mycobacterium</I>. Phenotypically, strain 01-305<SUP>T</SUP> was generally similar to <I>Mycobacterium triviale</I> ATCC 23292<SUP>T</SUP>, but some unique biochemical characteristics were observed. The 16S rRNA gene sequence of strain 01-305<SUP>T</SUP> was similar to those of <I>M. triviale</I> ATCC 23290 (GenBank accession no. AY734996, 99.9 % similarity) and <I>M. triviale</I> ATCC 23291 (AY734995, 99.9 %); however, it differed substantially from that of <I>M. triviale</I> ATCC 23292<SUP>T</SUP> (X88924, 98.2 %). Phylogenetic analysis based on 16S rRNA gene sequences placed strain 01-305<SUP>T</SUP> in the slow-growing <I>Mycobacterium</I> group close to <I>M. triviale</I> ATCC 23290 and <I>M. triviale</I> ATCC 23291, but not <I>M. triviale</I> ATCC 23292<SUP>T</SUP>. Unique mycolic acid profiles and phylogenetic analysis based on two different chronometer molecules, and the <I>hsp65</I> and <I>rpoB</I> genes, strongly supported the taxonomic status of this strain as representing a distinct species. These data support the conclusion that strain 01-305<SUP>T</SUP> represents a novel mycobacterial species, for which the name <I>Mycobacterium koreense</I> sp. nov. is proposed. The type strain is 01-305<SUP>T</SUP> ( = DSM 45576<SUP>T</SUP> = KCTC 19819<SUP>T</SUP>).</P>