In Vivo Chemosuppressive Effects of Kolaviron on 7,12-Dimethylbenzanthracene- Induced Mammary Lesions are Associated with Changes in Levels of Estrogen Receptor-a, CYP 1A1, Proinflammatory Cytokines, and Alterations to Metabolic Pathways Implicated in Ma

다국어 초록 (Multilingual Abstract)

Garcinia kola is a medicinal food commonly consumed in Sub-Sahara Africa, for which Kolaviron (KV) isthe active portion. As a follow-up to our earlier chemopreventive studies, we investigated the chemotherapeutic effects ofKV on experimentally induced mammary carcinogenesis in female Wistar rats. Mammary carcinogenesis was induced using80 mg/kg of 7,12-dimethylbenzanthracene (DMBA) administered by oral gavage. One hundred-fifty days post-DMBAinduction, estrogen receptor-a (ER-a) levels were determined in the experimental rats before treatment with KV commenced.
Treatment was done using 50, 100, and 200 mg/kg KV thrice a week for 4 weeks, after which the experiment was terminated.
Significantly higher levels of estrogen receptor-a, CYP 1A1, malondialdehyde, formation of lobular neoplastic cells,epithelial hyperplasia, lymphocyte infiltration, and increased cytokine (interleukin-6 and tumor necrosis factor-a) activitywere observed in DMBA-induced rats, which were attenuated in KV-treated rats. Tyrosine metabolism was exclusivelyenriched in DMBA-induced rats in contrast to KV-treated rats. Collectively, the results point to the chemotherapeutic potentialof KV.