Genetic Analysis of H7N7 Avian Influenza Virus Isolated From Waterfowl in South Korea in 2016

국문 초록 (Abstract)

A형 인플루엔자바이러스는 야생조류에 존재하며, 사람, 돼지, 가금 및 다른 포유류등 다양한 숙주를 감염한다. 본 연구에서는, 2016년 한국 서쪽의 철새도래지에서 채취한 철새 분변에서 재조합된 새로운 H7N7 조류인플루엔자를 분리하였으며 이 바이러스의 8개 유전자를 분석하였다. 분리된 A/waterfowl/Korea/S017/2016(H7N7) 바이러스의 유전자 분석 결과 이 바이러스는 야생조류 및 가금 오리에서 유래한 조류인플루엔자로 구성된 재조합된 유전자를 가지고 있었다. 계통 분석결과 이 바이러스는 유럽과 아시아계에 속하였다. 조류인플루엔자 바이러스가 계속 진화를 하고 H7 형 조류인플루엔자는 고 병원성 조류인플루엔자로 변하여 사람과 동물에게 커다란 위협이 될 수 있기에 계속 된 역학조사가 필요하다.

다국어 초록 (Multilingual Abstract)

Type A influenza virus is circulating in wild birds and can infect wide ranges of hosts such as humans, pigs, domestic birds, and other mammals. Many subtypes of avian influenza viruses are circulating in aquatic birds. Most avian influenza viruses found in aquatic birds are low pathogenic avian influenza viruses. Highly pathogenic avian influenza viruses have been found in waterfowls since 2005. It is known that H5 and H7 subtypes of avian influenza viruses can be mutated into highly pathogenic avian influenza viruses in domestic poultry. In this study, we isolated novel reassortant H7N7 avian influenza virus from the fecal materials of migratory birds in the Western part of South Korea in 2016, and analyzed the sequences of all its eight genes. The genetic analysis of our isolate, A/waterfowl/Korea/S017/2016 (H7N7) indicates that it was reassortant avian influenza virus containing genes of both avian influenza viruses of wild birds and domestic ducks. Phylogenetic analysis showed that our isolate belongs to Eurasian lineage of avian influenza virus. Since avian influenza viruses continue to evolve, and H7-subtype avian influenza virus can mutate into the highly pathogenic avian influenza viruses, which cause the great threat to humans and animals, we closely survey the infections in both wild birds, and domestic poultry, and mammals.

목차 (Table of Contents)

• Introduction
• Materials and Methods
• Results and Discussion
• References
• 초록

참고문헌 (Reference)

• 1 CDC, "Types of Influenza Viruses"
• 2 Fan, S., "Two amino acid residues in the matrix protein M1 contribute to the virulence difference of H5N1 avian influenza viruses in mice" 384 : 28-32, 2009
• 3 Hay, A. J., "The molecular basis of the specific anti-influenza action of amantadine" 4 : 3021-3024, 1985
• 4 Liu, M., "The influenza virus gene pool in a poultry market in South central china" 305 : 267-275, 2003
• 5 Jiao, P., "Single-amino-acid substitution in the NS1 protein changes the pathogenicity of H5N1 avian influenza viruses in mice" 82 : 1146-1154, 2008
• 6 Metreveli, G., "Phylogenetic analysis of the hemagglutinin gene of low pathogenic avian influenza virus H7N7 strains in mallards in Northern Europe" 54 : 453-456, 2009
• 7 Kim, C. M., "Pathogenicity and transmission studies of H7N7 avian influenza virus isolated from feces of magpie origin in chickens and magpie" 141 : 268-274, 2010
• 8 Belser, A. J., "Pathogenesis, transmissibility, and tropism of a highly pathogenic avian influenza A (H7N7) virus associated with human conjunctivitis in Italy, 2013" 216 : S508-S511, 2017
• 9 Horimoto, T., "Pandemic threat posed by avian influenza A viruses" 14 : 129-149, 2001
• 10 Wright, P. F., "Orthomyxoviruses;Fields Virology" Lippincott, Williams and Wilkins 1533-1579, 2001
• 11 Cui, P., "New influenza A (H7N7) viruses detected in live poultry markets in China" 499 : 165-169, 2016
• 12 McKimm-Breschkin, J. L., "Mutations in a conserved residue in the influenza virus neuraminidase active site decreases sensitivity to Neu5Ac2en-derived inhibitors" 72 : 2456-2462, 1998
• 13 Campitelli, L., "Molecular analysis of avian H7 influenza viruses circulatingin Eurasia in 1999-2005: detection of multiple reassortant virus genotypes" 89 : 48-59, 2008
• 14 Kawaoka, Y., "Is the gene pool of influenza viruses in shorebirds and gulls different from that in wild ducks?" 163 : 247-250, 1988
• 15 Nidom, C. A., "Influenza A (H5N1) viruses from pigs, Indonesia" 16 : 1515-1523, 2010
• 16 Webster, R. G., "Evolution and ecology of influenza A viruses" 56 : 152-179, 1992
• 17 Smietanka, K., "Evidence of persistence and multiple genetic modifications of H7N7 low-pathogenic avian influenza virus in wild mallards in Poland provided by phylogenetic studies" 40 : 131-138, 2011
• 18 Monne, I., "Emergence of a highly pathogenic avian influenza virus from a low-pathogenic progenitor" 88 : 4375-4388, 2014
• 19 Kurtz, J., "Avian influenza virus isolated from a woman with conjunctivitis" 348 : 901-902, 1996
• 20 Cardona, C., "Are live bird markets reservoirs of avian influenza?" 88 : 856-859, 2009