색소생산 및 색소비생산 Serratia marcescens 배양액에 의한 면역반응항진과 균의 항균제 및 인혈청에 대한 내성 = Enhancement of Immune Responses by Culture Filtrates from Pigmented and Nonpigmented Serratia marcescens and the Suceptibility of the Organisms to Antibiotics and Human Sera

다국어 초록 (Multilingual Abstract)

This study was undertaken to assess the susceptibility of pigmented and nonpigmented strains of Serratia marc escens to antibiotics and hurnan sera, and the effect of culture filtrates from pigmented and nonpigmented of Serratia marcescens on humoral and cellular immune responses in mice to thy- mus-depender.t and -indepependent antigens. Humoral immune response was measured by hemaggluti- nin (HA) and hemolysin (HE) to sheep red blood cell (SRBC), and Arthus or antibody response to pol- yvinylpyrrolidone (PVP). The cellular immune response was measured by delayed-type hypersensitivity (DTH) determined by footpad swelling reactin to SRBC.
The resistance of pigmented strains of Serratia marcescens to the bactericidal action of heat- inactiv- ated human serum was insignificantly greater than that of nonpigmented strains. However, the pigme- nted strains were significantly more resistant to the bactericidal action of heat-untreated human serum than that of nonpigmented strains.
The clinical isolates of Serratia marcescens was also tested for their resistance to several antibiotics, There was no difference between the pigmented and non-pigmented strains in the resistance to carbe- nicillin. However, nonpigmented strains were more resistant to gentamicin, kanamycin and tobramycin than the pigmented strains.
The intraperitoneal Administration of culture filtrates from the pigmented or nonpigmented strains into mice caused enhancemented of antibody response to SRBC or PVP, and of DTH to SRBC. Besides, their enhancement of immune responses was more prominent when culture filtrate from the pig- mented strains was administered.