녹차추출물이 에탄올 투여에 의한 초기 간 손상에 미치는 영향

국문 초록 (Abstract)

녹차 추출물이 에탄올에 의한 조기 간세포에 미치는 영향을 관찰하기 위해 흰쥐에 에탄올과 녹차 추출물을 투여하고 1시간 및 3시간 후 흰쥐의 간 글루타치온과 TNF-α량, 간항산화효소 활성도, 지질과산화물량 및 혈청 AST 및 ALT 활성도를 측정하였다. 또한 에탄올에 의한 만성 간 손상에
미치는 영향을 관찰하기 위해서 에탄올과 녹차 추출물을 2일 간격 투여 20일 후 흰쥐의 간 글루타치온과 TNF-α량, 간 항산화 효소활성도, 지질과산화물량 및 혈청 AST 및 ALT 활성도를 측정하였다. 간 글루타치온량은 대조군에 비하여 에탄올 투여군에서 1시간, 3시간 및 20일 경과 후에 모두 감소되었고, 에탄올과 녹차추출물 혼합투여로 간 글루 타치온량이 에탄올 투여군에 비하여 증가되어 녹차추출물이 에탄올에 의한 간 글루타치온량 감소를 억제시킴을 알 수 있다. 간 TNF-α량은 에탄올 투여 1시간, 3시간 및 20일 경과 후 증가되었고, 녹차추출물과 에탄올 혼합투여군은 에
탄올 투여군에 비하여 1시간, 3시간 및 20일 경과 후 모두 감소되어 녹차추출물 투여로 에탄올 투여에 의한 간조직의 TNF-α량 증가가 억제됨을 알 수 있다. 에탄올 투여군의 간 조직 TBARS량을 측정한 결과 에탄올을 투여한 후 1시간경과 및 3시간 경과 후 대조군과 유의한 차이가 없었다. 에탄올 투여군의 간 조직 항산화효소(SOD, catalase, GPx) 활성도를 측정한 결과 에탄올을 투여한 후 1시간 경과 및 3시간경과 후 대조군과 유의한 차이가 없었다. 에탄올 투여군의 혈청 ALT와 AST 활성도를 측정한 결과 에탄올을 투여한 후 1시간 경과 및 3시간 경과 후 대조군과 유의한 차이가 없었다. 이상의 실험 결과 녹차 추출물은 간에서 에탄올에 의해서 조기에 일어나는 글루타치온량 감소 및 TNF-α량증가를 억제시키는 작용이 있는 것을 알 수 있는데, 녹차 추출물의 이러한 효과는 녹차 추출물이 에탄올에 의한 간 손상을 억제하는데 중요한 작용을 할 수 있을 것으로 기대된다. 녹차 추출물에 함유된 에탄올에 의한 간 손상 방지 물질의 검색과 글루타치온 및 TNF-α에 대한 작용 기전 및 특성에 대한 연구가 계속된다면 우수한 간 손상 방지작용을 가진 물질이 개발될 수 있을 것으로 추측된다.

다국어 초록 (Multilingual Abstract)

The liver is the major target of ethanol toxicity and oxidative stress plays a role in development of alcoholic liver disease. This study was performed to investigate the effects of green tea extracts (GTE) on acute ethanol-induced hepatotoxicity in rats. Experimental animals were divided into 4 groups, control, GTE, ethanol, and GTE＋ethanol treatment, with 5 rats in each group. Ethanol (6 g/kg body weight (BW)) and GTE (200 mg/kg BW) were treated by gavage. At 1 hour, 3 hours and 20 days (6 g/kg BW every 2 days for total 10
doses) after ethanol and/or GTE treatments, animals were killed; hepatic tumor necrosis factor-alpha (TNF-α) and glutathione level, serum aspartate aminotransferase (AST), serum alanine aminotransferase (ALT), hepatic antioxidant enzymes (SOD, CAT, GPx) activities and hepatic thiobarbituric acid reactive substances (TBARS) were measured. At 1 hour and 3 hours, hepatic TNF-α levels were increased significantly in ethanol group and ethanol＋GTE group but that levels was significantly lower in ethanol＋GTE group compared with ethanol group. Hepatic glutathione level was decreased by ethanol treatment but GTE prevented the ethanol-induced glutathione decrement. The levels of liver marker enzymes (AST, ALT), liver antioxidant enzymes (SOD, CAT, GPx) and lipid peroxidation marker (TBARS) were not changed in rats of 1 and 3 hours after ethanol treatment. After 20 days, GTE decreased the changes of liver marker enzymes (AST, ALT) activities and TBARS level by ethanol. This study shows that GTE beneficially modulates TNF-α and glutathione levels in liver of ethanol administered rats. The GTE supplementation could be beneficial to liver by decreasing early changes of biomarkers of liver damage caused by ethanol.

목차 (Table of Contents)

• Abstract
• 서론
• 재료 및 방법
• 결과 및 고찰
• 요약
• 문헌

참고문헌 (Reference)

• 1 Hatano E, "Tumor necrosis factor signaling in hepatocyte apoptosis" 22 : S43-S44, 2007
• 2 Lee JS, "Supplementation of Pueraria radix water extract on changes of antioxidant enzymes and lipid profile in ethanol-treated rats" 347 : 121-128, 2004
• 3 Flohe L, "Superoxide dismutase assays. In: Methods in Enzymology, Vol 105" Academic Press 93-104, 1984
• 4 Song Z, "Silymarin protects against acute ethanol-induced hepatotoxicity in mice" 30 : 407-413, 2006
• 5 Lowry CH, "Protein measurement with folin phenol reagent" 193 : 256-277, 1951
• 6 Zhao J, "Protective effect of bicyclol on acute alcohol-induced liver injury in mice" 586 : 322-331, 2008
• 7 Augustyniak A, "Preventive action of green tea from changes in the liver antioxidant abilities of different aged rats intoxicated with ethanol" 21 : 925-932, 2005
• 8 Wu D, "Oxidative stress and alcoholic liver disease" 29 : 141-154, 2009
• 9 Buege JA, "Microsomal lipid peroxidation. In: Methods in Enzymology, Vol 52" Academic Press 302-310, 1978
• 10 Beltz LA, "Mechanisms of cancer prevention by green and black tea polyphenols" 6 : 389-406, 2006
• 11 Polavarapu R, "Increased lipid peroxidation and impaired antioxidant enzyme function is associated with pathological liver injury in experimental alcoholic liver disease in rats fed diets high in corn oil and fish oil" 27 : 1317-1323, 1998
• 12 Corns CM, "Herbal remedies and clinical biochemistry" 40 : 489-507, 2003
• 13 Tapsell LC, "Health benefits of herbs and spices: the past, the present, the future" 185 : S4-S24, 2006
• 14 Alschuler L, "Green tea: Healing tonic" 5 : 28-31, 1998
• 15 Ostrowska J, "Green tea protects against ethanol-induced lipid peroxidation in rat organs" 32 : 25-32, 2004
• 16 Lieber CS, "Ethanol metabolism, cirrhosis and alcoholism" 257 : 59-84, 1997
• 17 Dullo AG, "Efficacy of a green tea extract rich in catechin polyphenols and caffeine in increasing 24-h energy expenditure and fat oxidation and fat oxidation in humans" 70 : 1040-1045, 1999
• 18 Lee SI, "Effect of green tea and (-)-epigallocatechin gallate on ethanol-induced toxicity in HepG2 cells" 22 : 669-674, 2008
• 19 Pari L, "Effect of grape (Vitis vinifera L.) leaf extract on alcohol induced oxidative stress in rats" 46 : 1627-1634, 2008
• 20 Kumar RS, "Effect of Cassia auriculata leaf extract on lipids in rats with alcoholic liver injury" 11 : 157-163, 2002
• 21 Ding WX, "Dissection of the multiple mechanisms of TNF-alpha-induced apoptosis in liver injury" 8 : 445-454, 2004
• 22 Nanji AA, "Curcumin prevents alcohol-induced liver disease in rats by inhibiting the expression of NF-kappa B-dependent genes" 284 : G321-G327, 2002
• 23 Aebi H, "Catalase. In: Methods of Enzymatic Analysis, Vol 2" Academic Press 673-684, 1974
• 24 Kundu R, "Cajanus cajan Linn. (Leguminosae) prevents alcohol-induced rat liver damage and augments cytoprotective function" 118 : 440-447, 2008
• 25 Flohe L, "Assay of glutathione peroxidase. In: Methods in Enzymatic Analysis, Vol 105" Academic Press 114-121, 1984
• 26 Mcdonough KH, "Antioxidant nutrients and alcohol" 189 : 89-97, 2003
• 27 Willner IR, "Alcohol and the liver" 21 : 323-330, 2005
• 28 Nordmann R, "Alcohol and antioxidant systems" 29 : 513-522, 1994
• 29 Zhou Z, "A critical involvement of oxidative stress in acute alcohol-induced hepatic TNF-alpha production" 163 : 1137-1146, 2003