Interfacial control of polymeric nanowires for applications in 3D nano-printing and single cell engineering
저자
발행사항
포항 : 포항공과대학교 일반대학원, 2023
학위논문사항
학위논문(박사)-- 포항공과대학교 일반대학원 : 신소재공학과 2023. 8
발행연도
2023
작성언어
영어
발행국(도시)
경상북도
형태사항
; 26 cm
일반주기명
지도교수: 오승수
UCI식별코드
I804:47020-200000690132
소장기관
The interface of polymeric nanostructures plays an important role in controlling their shapes and mediating interactions with surrounding molecules. As the most of reactions involving in the polymer nano-architectures occur mainly at their interface, the fundamental understanding of the interfacial phenomena is essential to control their shape and functionalities. In particular, one-dimensional polymer nanowires with high aspect ratio have great availability as structural building units and mechanical/chemical/optical cues at nanoscale, raising the importance of interfacial regulation to control the properties of polymeric nanowires. However, the morphological and functional control of the polymeric nanowires with high physicochemical fragility remain exceptionally difficult due to the complexity of regulating the interfacial reactions during their fabrication and modification process.
In this dissertation, noble approaches to control nanoscale polymer interfaces are presented for applications in 3D nano-printing and single cell engineering. The key strategy is nanoscale fabrication and modification of polymeric nanowires via interfacial regulation that enhances shape controllability, mechanical stability, and optical manipulation capability. Unlike conventional approaches, the folding structures and molecular functions of diverse polymers can be preserved after 3D fabrication and surface modification of the nanowires, due to the mild fabrication conditions within ambient air at room temperature. Based on the functional preservation of intact polymers, spatially controlled growth of polymeric nanowires not only enables 3D printing of the nano-architectures with unique mechanical, biochemical, and optical properties, but also facilitates the development of the micromanipulators that can be utilized in cell modification. Therefore, this dissertation presents three-types of polymeric nanowire interface control strategies, classified as rheological, chemical and optical controls for applications in 3D nano-printing and single cell engineering.
First, for the first time, a type independent 3D nano-writing technique was developed by rheological control of ultra-shallow liquid interface. When a femtoliter volume of a biopolymer-containing aqueous solution is confined in an ultra-shallow liquid interface between the fine-tuned nanopipette and the desired printing position, solvent-exclusive evaporation and concurrent biopolymer solution supply occurs, leading to the direct formation of biopolymeric nanostructures. As the choice of biopolymer types does not influence on the solution confinement and solidification, the 3D nano-writing can be universally applicable to natural biopolymers without any modification. All kinds of biopolymers at hands, including nucleic acids, polysaccharides, and even recognitive/catalytic proteins, have proven to be easily printable in fabricating complex architectures with various 3D shapes (e.g., pillars and arches). Based on full understanding of the growth dynamics of confined biopolymers, 3D motor-operated precise nanopipette positioning enabled reproducible fabrication of nanowire patterns varying both the diameter (80 nm - 10 μm) and length (≥ 250 nm).
Second, pin-point localization of spatiotemporal biofunctions was achieved by surface-exclusive modification of protein nanowire arrays. Even after the nano-writing and subsequent surface-exclusive crosslinking, 3D-shaped biopolymers retained their inherent molecular functionalities, such as recognition of target molecules and acceleration of biochemical reactions. In this study, unprecedent experimental demonstration of spatially localized activation of protein functions was presented with several protein nanowire patterns, consisting of pure streptavidin, serum albumin, and horseradish peroxidase. Interestingly, the site-specifically printed protein nanopatterns conserved their unique target binding and antifouling abilities with no structural deformation in various solvents. The availability of enzyme nanowires was indeed striking; as localized chemical cues, horseradish peroxidase nanowires accelerated peroxidation reactions at nanoscale pin-point region with no crosstalk with adjacent non-enzymatic protein nanowires in a single batch.
Lastly, on-demand gene inoculator was developed for single cell modification by evanescent-field-driven release of genetic materials from nanowire surface. Immediate release (< 3.0 s) of pre-designed oligonucleotides on the surface of nanowire waveguide was successfully accomplished during insertion of the nanowire into a living cell. As the nanowire-based inoculator is mechanically robust and thin, it can readily penetrate the plasma membranes and subcellular envelops, enabling spatioselective gene delivery without cellular damage. In this work, the light propagation in the nanowire was also simulated, and it was revealed that the light-driven reaction is localized only on the near-field of nanowire surface due to the exponentially decaying evanescent field, minimizing optical damage to a cell. The on-demand modification of intracellular genes was successfully demonstrated through inducing the inhibition and expression of specific genes within an arbitrarily selected living cell. With the user-defined genetic regulation capability, the gene inoculation system holds great potential for cell engineering.
서지정보 내보내기(Export)
닫기소장기관 정보
닫기권호소장정보
닫기오류접수
닫기오류 접수 확인
닫기음성서비스 신청
닫기음성서비스 신청 확인
닫기이용약관
닫기학술연구정보서비스 이용약관 (2017년 1월 1일 ~ 현재 적용)
학술연구정보서비스(이하 RISS)는 정보주체의 자유와 권리 보호를 위해 「개인정보 보호법」 및 관계 법령이 정한 바를 준수하여, 적법하게 개인정보를 처리하고 안전하게 관리하고 있습니다. 이에 「개인정보 보호법」 제30조에 따라 정보주체에게 개인정보 처리에 관한 절차 및 기준을 안내하고, 이와 관련한 고충을 신속하고 원활하게 처리할 수 있도록 하기 위하여 다음과 같이 개인정보 처리방침을 수립·공개합니다.
주요 개인정보 처리 표시(라벨링)
목 차
3년
또는 회원탈퇴시까지5년
(「전자상거래 등에서의 소비자보호에 관한3년
(「전자상거래 등에서의 소비자보호에 관한2년
이상(개인정보보호위원회 : 개인정보의 안전성 확보조치 기준)개인정보파일의 명칭 | 운영근거 / 처리목적 | 개인정보파일에 기록되는 개인정보의 항목 | 보유기간 | |
---|---|---|---|---|
학술연구정보서비스 이용자 가입정보 파일 | 한국교육학술정보원법 | 필수 | ID, 비밀번호, 성명, 생년월일, 신분(직업구분), 이메일, 소속분야, 웹진메일 수신동의 여부 | 3년 또는 탈퇴시 |
선택 | 소속기관명, 소속도서관명, 학과/부서명, 학번/직원번호, 휴대전화, 주소 |
구분 | 담당자 | 연락처 |
---|---|---|
KERIS 개인정보 보호책임자 | 정보보호본부 김태우 | - 이메일 : lsy@keris.or.kr - 전화번호 : 053-714-0439 - 팩스번호 : 053-714-0195 |
KERIS 개인정보 보호담당자 | 개인정보보호부 이상엽 | |
RISS 개인정보 보호책임자 | 대학학술본부 장금연 | - 이메일 : giltizen@keris.or.kr - 전화번호 : 053-714-0149 - 팩스번호 : 053-714-0194 |
RISS 개인정보 보호담당자 | 학술진흥부 길원진 |
자동로그아웃 안내
닫기인증오류 안내
닫기귀하께서는 휴면계정 전환 후 1년동안 회원정보 수집 및 이용에 대한
재동의를 하지 않으신 관계로 개인정보가 삭제되었습니다.
(참조 : RISS 이용약관 및 개인정보처리방침)
신규회원으로 가입하여 이용 부탁 드리며, 추가 문의는 고객센터로 연락 바랍니다.
- 기존 아이디 재사용 불가
휴면계정 안내
RISS는 [표준개인정보 보호지침]에 따라 2년을 주기로 개인정보 수집·이용에 관하여 (재)동의를 받고 있으며, (재)동의를 하지 않을 경우, 휴면계정으로 전환됩니다.
(※ 휴면계정은 원문이용 및 복사/대출 서비스를 이용할 수 없습니다.)
휴면계정으로 전환된 후 1년간 회원정보 수집·이용에 대한 재동의를 하지 않을 경우, RISS에서 자동탈퇴 및 개인정보가 삭제처리 됩니다.
고객센터 1599-3122
ARS번호+1번(회원가입 및 정보수정)